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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Free Radic Biol Med. 2018 Feb 2;117:99–109. doi: 10.1016/j.freeradbiomed.2018.01.023

Figure 4. Overexpression of ECSOD and knock-down of xCT in PC3 cells shift intra-and extracellular redox states to oxidizing status.

Figure 4

(A) H2O2 level in the media using the Amplex Red assay. (B) Measurement of nitrite level in the media using Griess reaction assay. (C) Measurement of Intracellular ROS/RNS using H2DCFDA. (D) Redox potential values in the media based on Nernst equation calculation of Cys and CySS. *p-value ≤ 0.05 compared with the control.