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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Cell Microbiol. 2015 Jun 11;17(11):1640–1652. doi: 10.1111/cmi.12461

Figure 2. AnkA binds known and predicted MARs.

Figure 2

(A) EMSA with rAnkA and rSATB1 incubated with the matrix attachment region (MAR) of IGH (wt = 5’-TCTTTAATTTCTAATATATTTAGAAttc-3’) known to bind SATB1, and with a mutated IGH MAR (mut = 5’-TCTTTAATTTCTACTGCTTTAGAAttc-3’). The underscored nucleotides indicate the areas where AT changes were made. Because AnkA is degraded during purification (Figure S1), several bands are present in EMSA. (B) MARs within the CYBB promoter were predicted with MARFinder MAR-Wiz 1.5 (blue line) using default values; a high likelihood was assigned with a value greater than the 0.6 cut-off threshold. AnkA binding along the entire CYBB promoter and downstream positions was assessed by chromatin immunoprecipitation-quantitative PCR (ChIP-qPCR) (red bars).