Skip to main content
. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Cell Signal. 2018 Jan 11;45:1–11. doi: 10.1016/j.cellsig.2018.01.005

Fig. 5. Effect of inhibition of JNK signaling with SP600125 on LPA-mediated changes of the morphological and migratory phenotype of MCF10CA1a cells.

Fig. 5

MCF10CA1a cells were starved in serum reduced medium (0.1 % horse serum) starting 3 h before stimulation with LPA (1 µM). The JNK inhibitor SP600125 (10 µM) was added 30 min before stimulation of cells with LPA. A. Immunostaining of E-Cadherin and quantification of membrane and cytoplasmic signal. ANOVA / Sidak’s multiple comparison test. n = 30 cells per group, representative of 4 experiments. Scale bar applies to all images of this panel. B–E. Effect of SP600125 and LPA on edge length (Wilcoxon matched-pairs sign rank test, B), cell speed (paired t-test) and angular spread (paired t-test) (C), Cv(Δr) (D), and λ (Wilcoxon matched-pairs sign rank test, E). n= 13 experiments.