Skip to main content
. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Thromb Haemost. 2016 Jun 23;116(3):506–516. doi: 10.1160/TH15-11-0848

Figure 2. LARG plays a role in human, but not murine, megakaryocyte maturation.

Figure 2

(A) qRT-PCR on puromycin selected control or LARG shRNA (shRNA-1 used) knockdown in primary human MEP differentiated in vitro confirmed an over 80% knockdown of LARG. (B) Flow cytometric analysis for CD41 and CD42 expression shows a block in MK differentiation with LARG KD. Right bar plot is mean + SD of three independent experiments (p<0.05). (C-D) Flow cytometric analysis for CD41 and CD42 (left) and ploidy of gated CD41+ cells (right) in fresh (C) and in vitro differentiated (D) murine bone marrow cells shows no difference in megakaryocyte number, percentage, cell surface markers or ploidy in WT versus Larg KO. Representative data are shown (n=3 per genotype).