Skip to main content
. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Thromb Haemost. 2016 Jun 23;116(3):506–516. doi: 10.1160/TH15-11-0848

Figure 6. Larg KO platelets have compromised RhoA activation and myosin light chain phosphorylation upon thrombin and thromboxane stimulation.

Figure 6

(A) Detection of activated RhoA by G-LISA luminescence assay in washed platelets before (black) and after (gray) 1 min 0.01U/ml thrombin. Raw luminescence is shown. No difference is observed in basal (no agonist) RhoA activity between WT and Larg KO. (B) Detection of myosin light chain phosphorylation in platelet lysates after stimulation with different agonists. (Neg=No agonist; Thr=0.01U/ml thrombin; U46=1uM U46619; ADP (5uM); M=molecular weight marker). Image from one blot is shown with Larg part darkened to equalize GAPDH for ease of view. This does not affect quantitation shown in C. (C) Relative amount of phosphorylated (P)-MLC normalized to GAPDH for each experiment. Data for 4 separate experiments are graphed with the P-MLC level at baseline normalized to 1 for each experiment.