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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Toxicology. 2018 Feb 7;396-397:54–67. doi: 10.1016/j.tox.2018.02.004

Figure 2. Quantification of pokeweed mitogen (PWM)-induced splenocyte proliferation by treatment group and sex.

Figure 2

Female (A, C, E) and male (B, D, F) rats were administrated vehicle (VH, 0.3% aqueous carboxymethylcellulose), BPA or estrogen ethinyl estradiol (EE2) at the indicated dose level by oral gavage daily and sacrificed at postnatal day (PND) 90 (A, B), 6 month (C, D), and 1 year (E, F). Splenocytes were isolated and treated with PWM for 48 h after a 24 h pulse with [3H]-thymidine. Cells were harvested and quantified for [3H]-thymidine incorporation using a Tri-Carb 2100 TR scintillation counter, which is represented as counts per minute (CPM), Results are presented as mean ± SE. n = 2–10 rats/treatment group/sex, n = 2 rats in PND 1 year 250μg BPA/kg/day treated male group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 when compared to respective vehicle control (VH-Ov for female rats at PND 6 month) by a two way ANOVA with Dunnett’s posttest. For more details about VH-Ov, please see the Statistical Analysis section in the Materials and Methods.