Skip to main content
. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Toxicology. 2018 Feb 7;396-397:54–67. doi: 10.1016/j.tox.2018.02.004

Figure 5. Quantification of pokeweed mitogen (PWM)-induced IgM secretion by splenic B cells by treatment group and sex.

Figure 5

Male (A) and female (B) rats were administrated vehicle (VH, 0.3% aqueous carboxymethylcellulose), BPA or estrogen ethinyl estradiol (EE2) at the indicated dose level by oral gavage daily and sacrificed at postnatal day (PND) 90 day (A) and 6 month (B). Splenocytes were isolated and treated with PWM for 72 h. Post activation, supernatants were collected and the levels of secreted IgM were quantified by ELISA. Results are presented as mean ± SE. n = 2–10 rats/treatment group/sex, n = 2 rats in PWM treated 0.05μg EE/kg/day male group. * p < 0.05, ** p < 0.01 when compared to respective vehicle control (VH-Ov for female rats at PND 6 month) by a two way ANOVA with Dunnett’s posttest. For more details about VH-Ov, please see the Statistical Analysis section in the Materials and Methods.