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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Toxicology. 2018 Feb 7;396-397:54–67. doi: 10.1016/j.tox.2018.02.004

Figure 6. Percentage of CD25+ T cells post anti-CD3/28 activation by treatment group and sex.

Figure 6

Female (A) and male (B) rats were administrated vehicle (VH, 0.3% aqueous carboxymethylcellulose), BPA or estrogen ethinyl estradiol (EE2) at the indicated dose level by oral gavage daily and sacrificed at postnatal day (PND) 1 year (A) and 6 month (B). Splenocytes were isolated and treated with anti-CD3/28 for 48 h. The percentage of CD25+ cells within splenic T cells was quantified by flow cytometry. Results are presented as mean ± SE. n = 4–10 rats/treatment group/sex. * p < 0.05, ** p < 0.01, **** p < 0.0001 when compared to respective vehicle control by a two way ANOVA with Dunnett’s posttest.