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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Toxicology. 2018 Feb 7;396-397:54–67. doi: 10.1016/j.tox.2018.02.004

Figure 7. Quantification of the CD25 expression level on T cells post anti-CD3/28 activation by treatment group and sex.

Figure 7

Female (A, C) and male (B, D) rats were administrated vehicle (VH, 0.3% aqueous carboxymethylcellulose), BPA or estrogen ethinyl estradiol (EE2) at the indicated dose level by oral gavage daily and sacrificed at postnatal day (PND) 6 month (A, B) and 1 year (C, D). Splenocytes were isolated and treated with anti-CD3/28 for 48 h. The expression levels of CD25 on splenic T cells, represented as mean fluorescence intensity (MFI), were quantified by flow cytometry. Results are presented as mean ± SE. n = 2–10 rats/treatment group/sex, n = 2 rats in PND 1 year 250μg BPA/kg/day male groups. * p < 0.05, ** p < 0.01, **** p < 0.0001 when compared to respective vehicle control (VH-Ov for female rats at PND 6 month) by a two way ANOVA with Dunnett’s posttest. For more details about VH-Ov, please see the Statistical Analysis section in the Materials and Methods.