Figure 2.
Fluorescence imaging of Hsp90 inhibition-induced GFP-iNOS aggregation and clearance in transfected cells. GFP-iNOS fusion proteins were expressed in HEK293 and COS-7 cells. As shown, Hsp90 inhibition (Radicicol, 20 μM; GA, 5 μM) depleted GFP-iNOS. In the presence of proteasome inhibitor, iNOS aggregates were seen in cells. Representative images are shown from triplicate experiments.