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Viability, proliferation, and spreading behavior of myoblasts. A) Representative fluorescence images of Live/Dead staining for cell viability. B) F-actin/nuclei staining for cell spreading on fibers on day 7 postculture. C) Viability of myoblasts seeded on fibers on days 1, 3, and 7. D) Cell proliferation on fibers on days 1, 3, and 7 determined by PrestoBlue assay. Results are presented as means ± standard deviations (n = 3; *p < 0.05, **p < 0.01, and ***p < 0.001).
