Fig. 1.

Chronological life span variation in a natural yeast strain collection. a Representative chronological life span (CLS) assay, based on determination of the Survival Integral (SI), for the FY4 strain in our standard SC medium. b CLS determination for our natural strain collection in SC condition. Arrows indicate the parental strains of the “sake × tecc cross”. c Heatmap and boxplot representation of CLS variation within the natural strain collection in seven different conditions. The color gradient represents SI variation with dark and light green corresponding to long and short life span, respectively. Gray rectangles in the heatmap indicate no growth of the corresponding strains in the respective conditions. Strains depicted in red correspond to the parental strains of the “sake × tecc cross”