Fig. 2.

CLS variation across the “sake × tecc cross” reveals different aging QTLs depending on growth conditions. a Comparison of SIs between the parental strains of the “sake × tecc cross” shows significant differences; Asterisk, significantly different by Student’s t-test, p-value < 0.05. Values are mean ± SDs. b Violin plot representation of survival integrals determined for the “sake × tecc cross” segregants in the indicated conditions. c QTL mapping using Individual Segregant Analysis (ISA, left column) and Bulk Segregant Analysis (BSA, right column) strategies for CLS data obtained when strains were aged in SC medium containing 2% glucose (SC), 0.5% glucose (CR), 10% glucose (Glu10), and 2% galactose (Gal). For ISA, the dotted red line represents significance thresholds corresponding to p-values of 0.05. The black dashed line in the BSA panels, indicating the G′ statistic significance threshold, corresponds to a false discovery rate of 0.05