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Synthesis strategy of NFAP2 and its peptide fragments. (A) Two synthetic peptides, Fragment 2t (thioester form), and Fragment 1, were linked by native chemical ligation (Váradi et al., 2013) to produce full-length synthetic NFAP2. (B) The synthetized peptide fragments for functional mapping cover the full-length protein. Intramolecular disulphide bridges between cysteines are marked with orange lines and the γ-core motif in red letters.
