Table 1.
Amino acid sequence and in silico predicted physical and chemical properties of mature NFAP2 and its peptide fragments.
| Protein/peptide | Number of amino acids | Molecular weight (kDa) | Number of Cys | Number of Lys/Arg | Theoretical pI | Estimated charge at pH 7 | GRAVY |
|---|---|---|---|---|---|---|---|
| IATSPYYACNCPNNCKHKKGSGCKYHSGPSDKSKVISGKCEWQGGQLNCIAT | |||||||
| NFAP2 | 52 | 5.6 | 6 | 7/0/ | 9.01 | +5.2 | −0.731 |
| KYHSGPSDKSKVISGKC(-SH)EWQGGQLNC(-SH)IAT | |||||||
| Fragment 1 (Fr-1) | 29 | 3.1 | 2 | 4/0 | 8.79 | +2.1 | −0.752 |
| IATSPYYAC(-SH)NC(-SH)PNNC(-SH)KHKKGSGC(-SH)/SHUFFLE VARIANT: IAGAHKC(-SH)KPC(-SH)YGYKTNSC(-SH)C(-SH)NSPN | |||||||
| Fragment 2 (Fr-2, Sh-Fr-2) | 23 | 2.5 | 4 | 3/0 | 8.87 | +3.0 | −0.704 |
| GKC(-SH)EWQGGQLNC(-SH)IAT | |||||||
| Fragment 3 (Fr-3) | 15 | 1.6 | 2 | 1/0 | 5.99 | −0.2 | −0.373 |
| NNC(-SH)KHKKGSGC(-SH)/SHUFFLE VARIANT: C(-SH)C(-SH)NKGKNKGSH | |||||||
| Fragment 4 (Fr-4, Sh-Fr-4) | 11 | 1.2 | 2 | 3/0 | 9.39 | +3.1 | −1.682 |
| KYHSGPSDKSKVIS | |||||||
| Fragment 5 (Fr-5) | 14 | 1.5 | 0 | 3/0 | 9.53 | +2.1 | −1.157 |
| IATSPYYAC(-SH)NC(-SH)P | |||||||
| Fragment 6 (Fr-6) | 12 | 1.3 | 2 | 0/0 | 5.51 | −0.2 | +0.192 |
GRAVY, grand average of hydropathy value; NFAP2, Neosartorya fischeri antifungal protein 2.