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Expression of pro-inflammatory markers in male offspring gut. (A) Il-1β; (B) Il1r1; (C) Tnfα; (D) Tnfrsf1a; (E) Il-6; (F) Il6r; (G) Il-10; and (H) Tlr4 gene expression determined by RT-qPCR (n = 7/group). Data were analyzed by two-way analysis of variance, with post hoc Holm–Sidak tests for multiple comparisons. Data are expressed as mean ± SEM, where *P < 0.05 vs CD and +P < 0.05 vs HF.
