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. 2018 Mar 7;9:417. doi: 10.3389/fmicb.2018.00417

Table 1.

Molecular characterisation of Staphylococcus epidermidis isolates involved in CRBSIs.

Patientnumber Isolate number Type ofspecimen IS256 icaAB mecA qacA/B mecAcontrol ccrgroup mecgroup Combination of the ccr group and the mec group SCCmectype MLST
7 7.2 CVP + – + + + ND ND ND
7.3 + + + + + 4, 5 C 4,5C
7.4 BC + + + + + 3,5 A 3,5A
7.5 BC + + + + + 2 B 2B IV
7.6 BC + – + + + 2,5 B 2,5B
9 9.1 CVP + + + + + 5 A 5A
9.2 BC + + + + + 2 B 2B IV
12 12.1 CVP + + + + + 1 A 1A
12.3 BC + + + + + 1 A 1A
12.4 BC + + + + + 1 A 1A
12.5 BC + + + + + 1 A 1A ST54
14 14.1 CVP + + + + + 4 A 4A
14.2 AC + + + + + 3 A 3A III
14.3 BC + + + + + 3 A 3A III ST2
14.4 BC + + + + + 3,4 A ND
14.5 BC + + + + + ND A mec class A
18 18.1 BC – + + – + 4,5 A 4,5A
18.2 BC – + + – + ND A mec class A ST490
18.4 CVP – – + – + 2 B 2B IV
20 20.1 CVP + + + + + ND A mec class A
20.2 BC + + + + + 1,2,5 A 1,2,5A
22 22.1 CVP + + + + + 2 B 2B IV
22.2 BC + + + + + 2 B 2B IV
26 26.1 CVP + + + + + 2,5 B,C ND
26.2 BC + + + + + 2 B 2B IV ST28
27 27.1 CVP + + + + + 3 A 3A III
27.2 + + + + + 2,5 C 2,5C
27.3 BC + + + + + 3 A 3A III ST2
28 28.2 CVP + + + + + 2,4 B 2,4B
28.5 BC + + + + + 2 B 2B IV
30 30.1 CVP + + + + + ND B mec class B
30.3 BC + + + + + ND B mec class B ST2
36 36.1 AC + + + + + 2,4,5 B,C ND
36.2 BC + + + + + 4,5 C 4,5C
36.3 BC + + + + + 2 B 2B IV
38 38.1 CVP + – + – + 1,5 B 1,5B
38.2 BC – – + – + 1,5 B 1,5B ST596
38.3 BC + – + – + 1,5 B 1,5B
40 40.1 CVP + + + + + 4,5 C 4,5C
40.2 BC + + + + + 2 B 2B IV
40.3 BC + + + + + 2 B 2B IV
42 42.1 CVP + + + + + 2,5 B,C ND
42.5 BC – – + + + 2 B 2B IV
43 43.2 CVP + + + + + 2 B 2B IV
43.3 VC + + + + + 3 A 3A III
43.4 VC + + + + + 2 B 2B IV
43.5 BC + + + + + 2 B 2B IV ST2
43.6 BC + + + + + 4,5 C 4,5C
43.7 BC + + + + + 2 B 2B IV
44 44.1 CVP + + + + + 1 A 1A
44.2 BC + + + + + 1 A 1A ST54
53 53.1 CVP – – + – + 5 C 5C V
53.2 BC – – + – + 5 C 5C V ST59
53.3 BC – – + – + 5 C 5C V
59 59.1 VC + + + + + 2 B 2B IV
59.2 BC + + + + + 2 B 2B IV
60 60.1 CVP + + + + + 2 B 2B IV
60.2 BC – + + – + 2,5 B 2,5B
60.3 BC – – + – + 5 C 5C V

The VITEK® 2 automated system (bioMérieux, France), MALDI-TOF MS (matrix-assisted laser deabsorption time of flight mass spectrometry) and ID PCR all identified the collected isolates as Staphylococcus epidermidis; AC, arterial catheter; BC, blood culture; CVP, central venous catheter; MLST, multilocus sequence typing; M-PCR assay, multiplex polymerase chain reaction assay; ND, no data; SCCmec, Staphylococcal Chromosome Cassette mec; ST, sequence type; VC, VasCath line; +, detected by M-PCR assay; –, not detected by the M-PCR assay. The shaded areas under the SCCmec type column indicate that certain isolates (n = 32/59) had multiple or unusual ccr and mec gene complex combinations and in these cases the raw ccr and mec gene combinations, instead of the SCCmec type, were reported. A Roman numeral was therefore not assigned to novel ccr and mec gene complex combinations if it is discovered in other methicillin-resistant staphylococci.