Table 2.
Substrate specificity of racemase reaction of WT enzyme.
| Substrate | Direction |
|
|---|---|---|
| L → D | D → L | |
| Ala | 9.3 ± 0.3 | 9.3 ± 0.5 |
| Ser | 1.8 ± 0.1 | 1.8 ± 0.1 |
| Thr | 0.059 ± 0.002 | nd |
| Allo-Thr | 0.024 ± 0.001 | nd |
| Met | 1.9 ± 0.1 | 1.5 ± 0.1 |
| Glu | 0.055 ± 0.007 | 0.097 ± 0.064 |
| Trp | 0.22 ± 0.01 | 0.10 ± 0.03 |
| Tyr | Trace | Trace |
| Val | 2.5 ± 0.1 | 2.4 ± 0.1 |
| Leu | 0.13 ± 0.01 | 0.096 ± 0.005 |
| Asn | 0.71 ± 0.09 | 0.44 ± 0.01 |
| Gln | 0.57 ± 0.01 | 0.57 ± 0.01 |
| Lys | 0.92 ± 0.06 | 1.0 ± 0.1 |
| Arg | 7.6 ± 0.1 | 5.8 ± 0.1 |
| His | 1.4 ± 0.1 | 1.4 ± 0.1 |
The given numbers are the average of triplicate measurements and shown as specific activity (nmol/min/mg). Enzyme assay was performed under standard assay conditions. Other amino acids (Asp, Pro, Phe, Ile, and allo-Ile) were inert as substrate. nd, not detected.