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Kinetic parameters of racemase and β-lyase reactions.
| Reaction | Substrate | kcat (s-1) | Km (mM) | kcat/Km (s-1 mM-1) |
|---|---|---|---|---|
| Racemase | L-Ala | 2.31 ± 0.51 × 103 | 169 ± 27 | 13.7 |
| Racemase | D-Ala | 1.83 ± 0.15 × 103 | 150 ± 24 | 12.2 |
| β-Lyase | L-Cys | 9.30 ± 2.05 × 102 | 11.5 ± 1.1 | 80.9 |
Enzyme assay was performed at pH 10.0 and 40°C (racemase reaction) or 35°C (β-lyase reaction). Kinetic parameters were calculated with triplicate measurements.
