Figure 3.

TcOYE is expressed in replicative stages of Trypanosoma cruzi lifecycle. (A) TcOYE expression during the parasite lifecycle was evaluated by western blot with total protein extracts from different parasite stages and Vero cells (since trypomastigotes and amastigotes are derived from infected Vero cells, they were used as a cross-reactivity control), using rabbit TcOYE antiserum. Relative expression was estimated by densitometry normalized by tubulin expression. V, Vero cells, T, trypomastigotes, A, intracellular amastigotes, E, epimastigotes. (B) Kinetics of TcOYE expression during mammalian cells infective cycle. Parasites were followed by inmunocitolocalization with rabbit α-TcOYE polyclonal antibodies. Bar 10 µm (except 1–9 h 5 µm). (C) Confocal microphotographs showing TcOYE and TccTXNPx immunolocalization in different stages of Vero cells infection. Bar 10 µm.