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. 2018 Mar 7;9:456. doi: 10.3389/fimmu.2018.00456

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Copyright © 2018 Díaz-Viraqué, Chiribao, Trochine, González-Herrera, Castillo, Liempi, Kemmerling, Maya and Robello.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TcOYE overexpressing parasites presents higher levels of PGF_2α than control parasites. (A) Western blot analysis of total protein extracts from epimastigotes transfected with pTREX-n (empty vector) and pTREX-n TcOYE (includes complete TcOYE coding sequence) using rabbit α-TcOYE polyclonal antibodies. The relative expression was estimated by image densitometry analysis normalized by tubulin expression. TcOYE overexpression in epimastigotes was 3.4 times more than controls. (B) Trypomastigotes and extracellular amastigotes overexpression analyzed by IIF using rabbit α-TcOYE polyclonal antibodies. (C) Growth curves of TcOYE overexpressing parasites (pTREX-n TcOYE) vs. empty vector containing parasites (pTREX-n). (D,E) Determination of PGF_2α in epimastigotes and trypomastigotes. These results correspond to concentration values of PGF_2α from two replicates. Asterisks represent statistical significance (two tailed Student’s t-test).