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TcOYE overexpression increased cardiac tissue parasite load in infected mice. (A) Parasitemia progression in BALB/c mice infected with TcOYE overexpressing and control parasites. Parasitemia was measured every 3 days by microscopic examination of thin tail-blood smears. (B) Parasite load in cardiac tissue of mice infected with transfected Trypanosoma cruzi euthanized at the 30th dpi. N = 2–5. Asterisks represent statistical significance (two tailed Student’s t-test).
