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. 2018 Feb 16;9(3):250–255. doi: 10.1021/acsmedchemlett.7b00544

Figure 1.

Figure 1

Structures of 1a, 2v, and 5d, and the effects of new analogues on STAT3 DNA-binding activity in vitro. (A) Analogues 1a, 2v, and 5d; (B–G) EMSA analysis of STAT3 DNA-binding activity in nuclear extracts of equal total protein prepared from v-Src-transformed fibroblasts containing activated STAT3 preincubated with 0–100 μM of the indicated compounds for 30 min prior to incubation with the radiolabeled hSIE probe that binds STAT3. Positions of STAT3:DNA complexes in gel are labeled; control lanes (0) represent nuclear extracts pretreated with 0.05% DMSO. Data are representative of 2–3 independent determinations.