Binding mode prediction of 2f with hAChE (PDB id: 4EY7). (A) Compound 2f was shown
in stick mode colored in green. Key residues were labeled as thin
stick mode colored in orange. Intermolecular interactions were shown
with different colored dotted lines: light green, hydrophobic contact;
yellow, π-cation; purple, π-alkyl contact. (B) Lineweaver–Burk
plots resulting from subvelocity curves of AChE activity with different
substrate concentrations (25–450 μM) in the absence and
presence of 1, 5, 10, and 20 nM 2f.