Figure 1.
Ex vivo screen and transformation assays. (A) Ex vivo patient BPH tissues were transduced with negative control vector pLVX, positive control FLAG-p53, or FLAG-INGs. The expression of these genes was induced with doxycycline. Tissues were fixed and processed for staining by IHC for H3S10phos or FLAG. (B) Ex vivo patient benign tissues were transduced with control (pLVX) or FLAG-tagged ING3-expressing lentiviral particles. The expression of proliferation marker H3S10phos and ING3 was assessed by IHC. (C) Normal human fibroblast were transduced with pLVX or FLAG-ING3 and anchorage-independent growth assessed by colony-formation assays in methylcellulose. The transformation assays were performed in three independent experiments and combined in one graphic with representative pictures. (D) As in panel (C) but performed in 3T3-L1 cells. By convention, *P<0.05, ***P<0.001, and NS indicates not significant, as calculated by t-test. When not indicated, P-values are not significant. ING=INhibitor of Growth.