Figure 4.

ING3 regulates genes associated with cell cycle progression. (A) Gene ontology analysis of downregulated genes upon silencing of ING3. A major fraction of genes requiring ING3 for expression is involved in cell cycle regulation. For instance, the expression of cyclin A (CCNA2) is impaired in the absence of ING3. (B) The expression of CDKN1A assessed by real-time qPCR on reverse transcribed total RNA isolated from control (Scramble) or ING3-silenced (siING3) prostate (LNCaP) and breast (MCF7) cells. (C) As in (B) but for CDKN1B. (D) Proliferation of control (Scramble siRNA) or ING3-silenced (siING3 no.1 and siING3 no.2) LNCaP cells was monitored for 5 consecutive days by IncuCyte. Data were analysed using Wilcoxon signed-rank test. (E) Flow cytometric analysis of control (Scramble siRNA) or ING3-silenced (siING3 no.1 and siING3 no.2) LNCaP cells was conducted and (F) G₁/S ratio was calculated. (G) Control (Scramble siRNA) or ING3-silenced (siING3 no.1 and siING3 no.2) LNCaP cells were stained for Annexin V and analysed by flow cytometry. By convention, *P<0.05, **P<0.01, ***P<0.001, and NS indicates not significant, as calculated by t-test. When not indicated, P-values are not significant.