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. 2018 Jan 30;118(5):670–678. doi: 10.1038/bjc.2017.451

Figure 1.

Figure 1

Role of WNT5A in castrated mice (N=5 mice per group). (A) LNCaP cells with inducible WNT5A were subcutaneously injected into nude mice. All animals were castrated and divided to two groups. When WNT5A was induced by administering doxycycline via drinking water, growth of tumour was increased significantly. Error bars represent average±SE. N=5 per group. (B) There is no significant difference between Doxy (−) and Doxy (+) tumours in haematoxylin staining. (C) WNT5A, CCL2, and BMP6 mRNA were analysed from tissues using RT-PCR. When WNT5A was overexpressed, expression of CCL2 and BMP6 were also increased. (D) A single-picture representative of IF for CCL2 and F4/80. (E) Quantification of IF demonstrated that CCL2 levels in WNT5A overexpressing tumours are increased and macrophages (F4/80) are more infiltrated. Mean fluorescence intensity (MFI) was measured by Image J. Values are means+SE. N=5 per group. *P < 0.05. BMP6=bone morphogenetic protein 6; CCL2=chemokine ligand 2; MFI=mean fluorescence intensity.