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. 2018 Jan 30;118(5):670–678. doi: 10.1038/bjc.2017.451

Figure 2.

Figure 2

Induction of CCL2 expression in hormone sensitive CaP cells by WNT5A. (A) LNCaP and 22RV1 cells were treated with 0–100 ng/ml of WNT5A for 24 h. mRNA of CCL2 was increased. (B) The level of CCL2 protein was also increased by WNT5A. Upon treatment of WNT5A (100 ng/ml), CCL2 induction was increased at the (C) mRNA level and (D) protein level in a time-dependent manner. (E) A semi-quantitative RT-PCR revealed that cycloheximide (CHX) not actinomycin D (ActD) inhibited the expression of CCL2 by WNT5A in LNCaP cells. All study was repeated at least three times. *P < 0.05. CCL2=chemokine ligand 2.