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. Author manuscript; available in PMC: 2019 Mar 2.
Published in final edited form as: J Proteome Res. 2018 Feb 8;17(3):987–999. doi: 10.1021/acs.jproteome.7b00639

Figure 2. Glycosylation profiles of BG505 SOSIP.v4.1-GT1 and SOSIP.664 trimers.

Figure 2

HILIC-UPLC profiles of PNGase F-released and fluorescently-labelled N-linked glycans from BG505 SOSIP.v4.1-GT1 (left) and SOSIP.664 (right) trimers produced in CHO cells and purified by 2G12-affinity chromatography followed by SEC. Gp140 trimer (A & D); gp120 (B & E); gp41 (C & F). Oligomannose-type and hybrid glycans (green) were identified by their sensitivities to Endo H digestion. Peaks corresponding to complex glycans are depicted in pink. The pie charts summarize the quantification of the glycan processing states, as determined by integration of the chromatogram. The corresponding percentages are listed in Table S2. Glycan sequencing data derived by exoglycosidase digestion of the GT1 trimer are shown in Figure S1.

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