Figure 6.

Low-level overexpression of p53 promotes warfarin-induced epigenetic changes and Slug expression. A, nuclear protein levels of AcH3, AcH4, p300, CBP, CEBP-β, and Slug were assessed by Western blotting. B, relative protein levels were normalized to histone 3. C, recruitment of p53 to the slug promoter was assessed by ChIP assay with the indicated antibodies. D, transcriptional activation of slug by p53 was assessed with the luciferase assay. The data are shown as the means ± S.E. of triplicates and are representative of three independent experiments performed. *, p < 0.05; **, p < 0.01; ***, p < 0.001.