Figure 4.

Chitinase secretion of C. japonicus GH18 mutants. Strains were grown on a plate that contained MOPS with 1.5% agar, 2% colloidal chitin, and 0.2% glucose. After incubation at 30 °C for 5 days, the plates were stained with Congo red. This experiment was conducted in biological triplicate, and quantification of the clearing zones is shown in Table S2.