Figure 6.

muRif1 prefers G4 DNA much more than other structured DNA. EMSA was conducted on the T₆G₂₄ probe (5.6 nm) with the full-length muRif1 (2.7 nm) in the presence of various structured DNAs as competitors. Competitors used were dsDNA (lanes 3 and 4), Y-fork (lanes 5 and 6), flap (lanes 7 and 8), fork (lanes 9 and 10), cruciform (4WJ, lanes 11 and 12), telomere repeat (Tel-ds, lanes 13 and 14), and T₆(GA)₁₂ (lanes 15 and 16), all of which failed to compete out the muRif1 binding to T₆G₂₄ probe at 30-fold excess (×30). Lanes 17 and 18 contain cold T₆G₂₄ DNA as a competitor, and substantial competition was obvious even at 3-fold excess (×3). The relative amounts of unbound G4 probe (bands indicated by the vertical line to the left of panel) are shown in a bar graph at the bottom, with the level in the absence of muRif1 being taken as 100.