Figure 7.

High-affinity binding of muRif1 to subsets of parallel-stranded G4 DNA. A, EMSA was conducted on six parallel-type G4 DNA (CEB1, T95-T2, 93del, T₆G₂₄, G₁₅, TERC18), a hybrid-type G4 DNA (Telo23), and an RNA probe corresponding to TERC18 DNA. As a negative control, a T₆(GA)₁₂ probe was also assayed. 4.17 nm radiolabeled and heated oligonucleotide was run on each lane with none (lane 1), 0.17 nm (lane 2), or 0.5 nm (lane 3) purified muRif1. The radioactivity (cpm/pmol) of every probe was slightly different, which is indicated in Fig. S7. B, fraction of the Rif1-bound probe was normalized and presented as a bar graph. C–E, EMSA was performed as above with partially-purified muRif1-NC (C), muRif1-NTD (D), or muRif1-CTD (E). The amount of the bound probe was calculated as in B and presented as bar graphs. See Fig. S7 for raw gel images of EMSA with muRif1-NC, -NTD, and -CTD.