Table 1.

Dissociation constant (K_d) for interaction between T₆G₂₄ G4-probe and muRif1 proteins

Probe concentration	8.33 nm	16.7 nma	38.9 nma
muRif1 (full-length, if n = 2)	0.197 nmb	0.256 nmb	0.145 nmb
muRif1-NC (if n = 1)	8.68 nm	4.51 nm	1.45 nm
muRif1-NC (if n = 2)	20.7 nmb	12.9 nmb	11.5 nmb
muRif1-NTD	6.49 nmc (6.74 nmd)	2.35 nm	1.51 nm
muRif1-CTD	33.0 nmc (87.4 nmd)	61.9 nm	18.1 nm

^aData are from Fig. S4.

^bK_d value is calculated assuming that the two binding sites have equal affinity to the G4-probe.

^cData are from Fig. 5A.

^dData are from Fig. 5B.