Table 2.
DNA oligonucleotides used in this study
DNA substrates for EMSA (Fig. 7C) were prepared by annealing: (H1 + H5), dsDNA; (H1 + H4), Y-fork; (H1 + H3.5 + H4), flap; (H1 + H2.5 + H3.5 + H4), fork; (H1 + H2 + H3 + H4), 4WJ; (TelG-Fw,+ TelC-Rv),Tel-ds.
| Oligonucleotide name | Length | Sequence (5′-to-3′) | Refs. |
|---|---|---|---|
| G-quadruplex-forming oligonucleotides | |||
| T6G24 | 30-mer | TTTTTTGGGGGGGGGGGGGGGGGGGGGGGG | This study |
| T2G15T | 18-mer | TTGGGGGGGGGGGGGGGT | 38 |
| VEGF | 22-mer | CGGGGCGGGCCTTGGGCGGGGT | 39 |
| c-kit2 | 21-mer | CGGGCGGGCTCGAGGGAGGGT | 40 |
| c-myc | 22-mer | TGAGGGTGGGTAGGGTGGGTAA | 41 |
| Telo24 | 24-mer | TTAGGGTTAGGGTTAGGGTTAGGG | 42 |
| Thrombin aptamer | 15-mer | GGTTGGTGTGGTTGG | 43 |
| Telo22 | 22-mer | GGGTTAGGGTTAGGGTTAGGGT | 44 |
| bcl-2 | 23-mer | GGGCGCGGGAGGAATTGGGCGGG | 45 |
| Telo23 | 23-mer | TAGGGTTAGGGTTAGGGTTAGGG | 46 |
| Telo25 | 25-mer | TAGGGTTAGGGTTAGGGTTAGGGTT | 46 |
| CEB1 | 18-mer | AGGGGGGAGGGAGGGTGG | 55 |
| T95–2T | 18-mer | TTGGGTGGGTGGGTGGGT | 47 |
| 93del | 16-mer | GGGGTGGGAGGAGGGT | 56 |
| G15 | 15-mer | GGGGGGGGGGGGGGG | This study |
| TERC18 | 18-mer | GGGTTGCGGAGGGTGGGC | 47 |
| Other DNA oligonucleotides | |||
| T6(GA)12 | TTTTTTGAGAGAGAGAGAGAGAGAGAGAGA | This study | |
| TelG-Fw | TTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGG | This study | |
| TelC-Rv | CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAA | This study | |
| sub01(H1) | GTGACCGTCTCCGGGAGCTGGAAACGCGCGAGACGAAAGG | 26 | |
| sub05(H5) | CCTTTCGTCTCGCGCGTTTCCAGCTCCCGGAGACGGTCAC | 26 | |
| sub04(H4) | CGAGTTGCTCTTGCCCGGCGCAGCTCCCGGAGACGGTCAC | 26 | |
| sub07(H3.5) | CGCCGGGCAAGAGCAACTCG | 26 | |
| sub06(H2.5) | CCTTTCGTCTCGCGCGTTTC | 26 | |
| sub02(H2) | CCTTTCGTCTCGCGCGTTTCGCCAGCCCCGACACCCGCCA | 26 | |
| sub03(H3) | TGGCGGGTGTCGGGGCTGGCCGCCGGGCAAGAGCAACTCG | 26 | |