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CD16a N-glycan composition impacts polypeptide structure. Select backbone amide cross-peaks observed in an HSQC experiment using CD16a expressed with either complex-type (red contours) or oligomannose (black contours) N-glycans. One peak present in the spectrum of CD16a with complex-type N-glycans but absent in the oligomannose form is indicated (†). Two peaks appearing in the spectrum with oligomannose N-glycans are indicated (*).
