Figure 3.

CNFy cargo is universally delivered by CNF1, CNF2, and CNF3 delivery domains. HEK293-T cells with reporter plasmids were treated with the indicated toxin at the indicated doses and subjected to SRE-luciferase assay as described under “Experimental procedures”. -Fold activation values are in comparison with untreated cells. Normalization of -fold activation is to the maximum activation of CNF1 as determined by the four-parameter logistic equation for each experiment. Results shown are from three independent experiments where each data point was performed in triplicate. Data points shown are the mean values for that specified dose. Error bars represent the S.D. Composition of each chimeric toxin is shown above the corresponding plot. Corresponding scatter plots of all data points used to derive the best fit lines and mean values are shown in Fig. S4. A, dose-response curve comparing CNFy cargo delivered by CNF1 delivery vehicle (CNF1, green closed triangles; CNFy, red closed squares; CNF1y-720, light green open triangles; CNF1y-735, dark green open triangles). B, dose-response curve comparing CNFy cargo delivered by CNF2 delivery vehicle (CNF1, green closed triangles; CNFy, red closed squares; CNF2y-688, yellow open circles; CNF2y-720, light blue open circles; CNF2y-735 dark blue open circles). C, dose-response curve comparing CNFy cargo delivered by CNF3 delivery vehicle (CNF1, green closed triangles; CNFy, red closed squares; CNF3y-688, purple open diamonds; CNF3y-735, pink open diamonds). D, CNF3 cargo is delivered less efficiently by CNF1, CNF2, and CNFy delivery vehicles. Experiments were performed similarly as in A–C. Dose-response curve comparing CNF3 cargo delivered by CNF1, CNF2, and CNFy delivery vehicles (CNF1, green closed triangles; CNF3, purple closed diamonds; CNF13-735, green open triangles; CNF23-735, blue open circles; CNFy3-735, red open squares).