Table 1.
Effective concentrations of CNF toxins
| Toxin | EC50 | EC50 ratioa |
|---|---|---|
| nm | ||
| CNF1 | 0.018 ± 0.008 | 1 |
| CNFy1-688 | 0.068 ± 0.023 | 3.8 |
| CNFy1-720 | >26b | >1444b |
| CNFy1-735 | >6.7b | >372b |
| CNF31-688 | 0.031 ± 0.009 | 1.7 |
| CNF31-735 | 0.071 ± 0.026 | 3.9 |
| CNF121-688 | 0.088 ± 0.049 | 4.9 |
| CNF121-720 | 0.079 ± 0.034 | 4.4 |
| CNF1y1-688 | NDc | NDc |
| CNF1y1-720 | NDc | NDc |
| CNF1y1-735 | NDc | NDc |
| CNF21-688 | NDd | NDd |
| CNF21-720 | NDd | NDd |
| CNFy | 0.25 ± 0.19 | 1 |
| CNF1y-720 | 4.5 ± 4.0 | 18 |
| CNF1y-735 | 0.28 ± 0.09 | 1.1 |
| CNF2y-688 | 2.5 ± 0.5 | 10.0 |
| CNF2y-720 | 2.4 ± 1.0 | 9.6 |
| CNF2y-735 | 0.99 ± 0.29 | 4.0 |
| CNF3y-688 | 0.076 ± 0.037 | 0.3 |
| CNF3y-735 | 0.82 ± 0.31 | 3.3 |
| CNF1y-223 | 1.8 ± 1.7 | 7.2 |
| CNF1y1y | NDc | NDc |
| CNF1y-688 | NDd | NDd |
| CNF2 | 0.084 ± 0.031 | 1 |
| CNFy2-688 | 2.7 ± 1.6 | 32 |
| CNFy2-720 | 62 ± 28 | 738 |
| CNFy2-735 | 2.8 ± 1.6 | 33 |
| CNF32-688 | 0.19 ± 0.08 | 2.3 |
| CNF32-735 | 0.029 ± 0.013 | 0.3 |
| CNF12-688 | NDd | NDd |
| CNF12-720 | NDd | NDd |
| CNF3 | 0.028 ± 0.015 | 1 |
| CNF13-688 | 6.3 ± 1.4 | 225 |
| CNF13-735 | 1.2 ± 0.6 | 43 |
| CNF23-688 | 0.93 ± 0.85 | 33 |
| CNF23-735 | 0.67 ± 0.25 | 24 |
| CNFy3-688 | 8.2 ± 2.5 | 293 |
| CNFy3-735 | 0.59 ± 0.39 | 21 |
a The EC50 ratio was determined by dividing the EC50 of the chimera by that of the native CNF toxin with the same cargo domain A.
b The EC50 values are estimated, but the errors could not be determined.
c Not determined; soluble protein was obtained, but maximum activation could not be determined.
d Not determined; protein was insoluble and could not be purified.