iTRAQ-based relative ratios of peptides/proteins identified by GST pulldown
Cross-comparison ratios of iTRAQ-labeled peptides were determined by ProteinPilot software and analyzed using MATLAB. Ratios are averaged across 4 biological replicates. A protein had to show the same deregulation trend in at least 2 of the 4 replicates to be considered as deregulated with a p value <0.05. Ratios of >1.2 for increased interactions or <0.82 for reduced interactions were used as cut-off from our previous work using iTRAQ (26).