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. 2018 Jan 23;293(10):3770–3779. doi: 10.1074/jbc.M117.810168

Figure 3.

Figure 3.

VNUT is involved in ATP release from mouse neutrophils. The data are shown as the mean ± S.E. A and B, the release of ATP (left panels), MPO (middle panels), and MMP-9 (right panels) from bone marrow-derived neutrophils from wildtype (open bars) and VNUT−/− mice (filled bars) was assessed 3 min (ATP) or 20 min (MPO and MMP-9) after the addition of 5 μm A23187 (A) and 100 nm W-peptide (B, n = 4–10). C, effects of 0.1 or 1 μm clodronate on A23187-induced ATP release from mouse neutrophils. D, temperature dependence for A23187-induced ATP release (n = 8–9). E, calcium dependence for A23187-induced ATP release. Mouse neutrophils were incubated in the presence or absence of 1 mm EGTA (left panel) and 50 μm EGTA-AM (right panel) (n = 7–10). N.D., not detected; N.S., not significant; **, p < 0.01.