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Multiple components of CRISPR/Cas9 system are delivered into cells to achieve a specific function. (a) Cas9 and sgRNA for gene disruption (knock-out), (b) Cas9, sgRNA, and a template ssDNA for mutation correction, (c) Cas9, sgRNA, and a template DNA for gene insertion (knock-in), and (d) Cas9 and two sgRNAs for gene deletion.
