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Comparison, and pros/cons of CRISPR/Cas9 delivery in different formats. Compared to other format of delivery, Cas9-RNP delivery has the most advantage as it offers no insertional mutagenesis, high editing efficiency, low off-target, and low immunogenicity.
| Cas9 delivery in different formats | ||||
|---|---|---|---|---|
| Viral | Plasmid | RNA | Protein | |
| Insertional mutagenesis | High | Moderate | No | No |
| Editing efficiency | High | Moderate | Moderate | High |
| Off-target | Low | Moderate | Moderate | High |
| Immunogenicity | High | Moderate | Moderate | Low |
