Figure 2.
a) Catalysis of nanozymes with different chemical headgroups in neutral pH for 2 hours at 37 ºC. b) Zeta potential of NZ1-3 (1 μM) measured at range of pH (3.5–7.4) is plotted against different pH values. Error bars represent standard deviations based on three independent measurements per pH value. c) Nanoparticle and catalyst diffusion into P. aeruginosa (CD-1006) biofilms after incubation for 1 hour in pH 7.4 media with NZ 1-3 (400 nM), as measured by ICP-MS. d) Confocal images of biofilm incubated with nanozymes (1-hour, 400 nM) followed by incubation with alloc-Rho (1 hour, 100 μM), Biofilm control is the negative control in absence of nanozyme. e) Quantitative analysis of fluorescence intensity generated upon addition of different nanozymes.