Figure 2.

a) NIRFI of PaIr monomer and PaIr NPs. b) Thermal images and c) Quantitative temperature variations of PaIr NPs and PaIr monomer. d) ROS generations of PaIr NPs. PaIr NPs were irradiated by NIR laser (680 nm) at 0.3 w/cm² for 3 min. e) Accumulated drug release of PaIr NPs in pH 7.4 and pH 5.0, with or without laser treatments. f) Blue fluorescence recovery of Ir indicated drug release of PaIr NPs. The image was captured under 365 nm UV lamp. “Pre” denoted the control samples before laser treatments (fresh made). “0” denoted non-laser treated samples, and “L” and “H” corresponded to low and high laser powers respectively. Cell viabilities of PaIr NPs towards U87-MG tumor cells g) without or h) with laser treatments. i) Combination index (CI) illustrated the synergistic effect of phototherapy and chemotherapy. CI versus Fraction affected (Fa) plot was calculated based on Figure 2h. j) FACS quantitatively exhibited the ROS production of PaIr NPs in U87-MG tumor cells. The ROS production indicated by a ROS indicator, 2′,7′-dichlorofluorescin diacetate (DCF-DA), Pa denoted the fluorescence of PaIr NPs. k) Light-triggered controllable and precise cancer therapy in cellular level. 3,3′-Dihexyloxacarbocyanine Iodide, DIOC(6) indicates live cells (green), Propidium iodide (PI) stains dead cells (red). The laser treated areas were marked with ‘L’. The scale bar is 200 μm.