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The primer sequences for each primer used in the real-time RT-PCR. The primer sequences and annealing temperature of IL-1β, TNF-α, MMP-13, and α-tubulin.
| Genes | Primer sequences | Annealing temperature (°C) |
|---|---|---|
| α-Tubulin | Forward: GCTGTGGTTGAGCCCTACAAT | 58 |
| Reverse: GTAGGTTGGGCGCTCAATGT | ||
| IL-1β | Forward: TTGTTGAGCCAGGCCTCTCT | 58 |
| Reverse: CCAAATGTGGCCGTGGTT | ||
| MMP-13 | Forward: CCCTCTGGTCTGTTGGCTCAC | 58 |
| Reverse: CTGGCGTTTTGGGATGTTTAGA | ||
| TNF-α | Forward: GGCTCAGAATCAGACCTCAG | 58 |
| Reverse: GCTCCACATTGCAGAGAAGA |
