Table 1.
Target | Primer name | Sequence (5′-3′) | Bases | Final amplicon size |
---|---|---|---|---|
SAM-1 | F3 | GAGGATGGGGTGCTCATGG | 19 | 220 bp |
B3 | CCTTATTAACTATCTCCAGYAG | 22 | ||
FIP | GACTTTGCAACAAGYCTTGCCAGCATTTAGCGGGAAAGATG | 41 | ||
BIP | ATTGGAATAGCAARGCCTTTATCGTCATTATACCCATCTTTCGC | 44 | ||
LF | CRCCTGAYCTATCTACTTTAG | 21 | ||
LB | CTRTATATTAATACATTTGGCAC | 23 | ||
SF | TACACAAKCCAGAAAAGACG | 20 | ||
SB | TGTTTGGTRCAGGTTTCATATG | 20 | ||
Probe | CTTGTGTAGCAGATGTTTGGTACAGG |
26 |