Figure 3.

Morphology of Hob using hematoxylin-eosin (HE) staining (a) and SEM (b, c) after 4 days of culture and expression of BSPII (d, e) and osteopontin (f, g) in human primary osteoblasts after 14 days culture on PCL scaffold (d, f) and on BMP-2/Ibu bifunctionalized scaffold (e, g). Cells are well spread and migrate along nanofibers (a–c). The nuclei were labeled with DAPI and actin with phalloidin. BMP-2: bone morphogenic protein 2, BSPII: bone sialoprotein 2, Ibu: Ibuprofen, and Osp: osteopontin.