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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Sci Transl Med. 2017 Jul 12;9(398):eaal1243. doi: 10.1126/scitranslmed.aal1243

Fig. 6. Zbtb7a expression is essential in elicitation of AM-directed humoral and cellular alloimmunity.

Fig. 6

Allogeneic reconstitution of AM was by intrabronchial transfer of 4×105 huCD68-Kd Tg C57BL/6 AM into C57BL/6 recipients. Development of H-2Kd specific Abs in BAL fluid (A) and lung-infiltrating Th17 cells in lungs (B) were measured respectively by flow cytometry and ELISPOT at 15 weeks by our earlier described protocol (36). Combinations of donor and recipient Zbtb7a knockdown were evaluated. Representative anti-H-2Kd titers (in sTable 3) are presented as overlaid histograms (n=5–7) and individual MFI values are indicated. ELISPOT assay measured alloreactive (H-2Kd specific) IL-17 producing cells per million lung leukocytes (mean±SEM) and one-way ANOVA was applied to test statistical significance (*).