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. Author manuscript; available in PMC: 2018 Mar 12.
Published in final edited form as: Sci Transl Med. 2017 Jul 12;9(398):eaal1243. doi: 10.1126/scitranslmed.aal1243

Fig. 7. Zbtb7a deficiency in donor AMs reduces donor-derived alloexosome production.

Fig. 7

(A) Quantitation of total exosomes isolated from cell-free BAL fluid following adoptive transfer of allogeneic AMs from Fig. 6. Data from three mice per AM transfer group were individually analyzed and presented as mean±SEM. One-way ANOVA was applied with Brown-Forsythe test that compares exosomes from the AM transfer groups with those from C57BL/6 as the baseline expression. Significance (p values) of differences is indicated. (B) Flow cytometric analysis of the BAL-derived exosomes was performed by anti-CD63 coated Exo-Flow magnetic beads. Exosomes bound to beads were positively identified by staining with Exo-APC. The AM-derived exosomes (Siglec-F+) were analyzed for preponderance of donor (for being H-2Kd+ and H-2Kb+) or recipient (for being H-2Kd− and H-2Kb+) origin of BAL exosomes.