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Labeling specificity of Click reaction. Increased concentration of alkyne-Alexa Fluor 488 increases fluorescent signal intensity of nanoparticle aggregates in nanoparticle-treated cells (right hand panels), while increased concentration of nanoparticles leads to greater nanoparticle uptake (bottom right). Cells not treated with nanoparticles (left panels) have low background Alexa Fluor 488 signal under the same staining and imaging conditions.
